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Objective To investigate the current situation on the infection of Acinetobacter baumannii in ICU and evaluate the efficiency of resistance control project which was performed in Xiaolan hospital,so as to provide scientific basis for clinical rational use of antibiotics.Methods Used VITEK-2 microbiology analysis system for the pathogen identification and drug sensitivity.Compared drug resistance of Acinetobacter baumannii isolated from the samples of ICU patients in 2012 (before the project executed)with that in 2013 (after the project executed).Results 71 strains and 57strains Acinetobacter bauman-nii were isolated from the ICU in 2012 (before the project executed)and in 2013 (after the project executed)respectively, which majorly isolated from the samples of respiratory system,and occupied 94.4% and 84.2% respectively.The infection rate of multi-resistant Acinetobacter baumannii was decreased from 77.5% to 66.7%,and the resistance rate of Acinetobact-er baumannii to cefepime,ceftazidime,cefatriaxone,tobramycin and cotrimoxazole decreased distinctly after the implementa-tion of drug resistance control management (χ2 > 3.84,P <0.05).Conclusion The drug resistance rates of Acinetobacter baumannii to 14 kinds of routine antibacterial drugs in 2013 were lower than that in 2012 in ICU.Among them,the drug re-sistance rates of Acinetobacter baumannii to 5 kinds of antibacterial drugs decreased distinctly.
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Objective To investigate the relationship of sperm morphology with seminal plasma zinc and sperm acrosin activity in male infertile patients.Methods 455 patients with male infertility were divided into 5 groups and 8 groups according to age and sperm morphology respectively.The sperm morphology,seminal plasma zinc and sperm acrosin activity were detected by the Diff-Quik stain,modified 5-Br-PAPS and modified Kennedy methods respectively.Results The percentages of morphologically normal sperm and seminal plasma zinc concentration in the infertile men groups were not different in various age groups(P >0.05 ).The sperm acrosin activity in the age group of 36-50 years was remarkably decreased (P 0.05).Seminal plasma zinc concentration in the infertile men groups were not different in the 8 groups (P >0.05 ).Sperm acrosin activity was decreased with the percentage decrease of morphologically normal sperm (r =0.93,P <0.01).Conclusion The percentage of morphologically normal sperm is not affectd by the age and the seminal plasma zinc,which is positively correlated with the sperm acrosin activity.
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<p><b>OBJECTIVE</b>To investigate the effects of experimental varicocele on mitochondria calcium and cytochrome C of the epididymal cells in adolescent rats.</p><p><b>METHODS</b>Forty male adult Wistar rats were divided into two groups randomly: varicocele group (VG) and sham operation group (SOG) by partial ligation or exposure of the left renal vein. Bilateral epididymides were removed after ten</p><p><b>RESULTS</b>The content of mitochondria weeks. Mitochondria calcium and cytochrome C levels of the epididymal cells were detected. calcium decreased (P < 0.001 ) while that of cytochrome C increased (P < 0.05) markedly in the experimental group compared with SOG.</p><p><b>CONCLUSION</b>Calcium dyshomeostasis and mitochondrial damage of the epididymal cells caused by varicocele may play an important role in leading to subfertility.</p>
Subject(s)
Animals , Male , Rats , Calcium , Metabolism , Cytochromes c , Epididymis , Metabolism , Infertility, Male , Mitochondria , Metabolism , Rats, Wistar , Varicocele , MetabolismABSTRACT
Objective Direct amplification of length polymorphism (DALP) as a new molecular marker was used to establish a set of stable DALP reaction system for the plants of Rhodiola L. Methods Some significant parameters of DALP reaction procedure were investigated and optimized by taking the DNA genome for the plants of Rhodiola L. as template. Results The reaction system was : 20 ?L reaction system containing 2. 5 mmol/L Mg2+ , 1. 25 mmol/L dNTPs, 60 ng DNA template, 1 ?L 5 pmol/L selective primer, 3 ?L 5 pmol/L reverse primer, selective primer: reverse primer is 1 : 3, and 2 U Taq DNA polymerase. Amplification program is 95℃ pre-denatured for 5 min, 94℃ denatured for 30 s, 50℃ annealed for 30 s, 72℃ extending for 1 min; after 30 cycles, and then 72℃ extending again for 10 min to the end of PCR reaction. Conclusion This DALP reaction system is efficient to identify the species and local populations for the plants of Rhodiola L. repeatedly with the stronger stability and reliability.
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AIM:To investigate the role of Shengmai Powder (Radix Ginseng, Radix Ophiopogonis and Fructus schisandrae) (SMP) in treatmen of hemorrhagic shock in rabbits. METHODS: The concentrations of endothelin (ET), tumor necrosis factor (TNF), ? endorphin (? EP) and nitric oxide (NO), the activity of nitric oxide synthase (NOS) in plasma were respectively tested by different biochemical assays in hemorrhagic shock in rabbits. RESULTS: SMP could obviously decrease the ET,TNF,NO level and NOS activity ( P
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AIM: To study the cell apoptosis and the change of microstructure and ultrastructure in epididymis with experimental varicocele (EVC) in rats. METHODS: Experimental varicocele model was induced by partial ligation of the left renal vein in adolescent Sprague-Dawley Wistar rats. Apoptotic cells were detected by in situ terminal deoxynucleotityl transferase-mediated dTUP nick end labeling (TUNEL) technique. The corpus epididymis of the rats was prepared for light and electron microscopic observation. The microstructure and ultrastructure of the epididymis were studied. RESULTS: There was certain proportion of apoptosis cells in epididymis cells in control rats. The incidence of apoptosis increased remarkably in experimental group than that in control group (P
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AIM: To observe the effect of ischemic preconditioning on contents of cytochrome C and mitochondrial calcium in rats after focal cerebral ischemic-reperfusion. METHODS: Focal cerebral ischemic model was made by occlusion of right middle artery in Wistar rats (ischemia for 2 h and reperfusion for 4 h). Rats were randomly divided into three groups: ischemia pretreatment, model and sham operation. Rats in ischemic pretreatment group were undergone transient ischemic preconditioning (30 min) and reperfusion (72 h). The contents of cytochrome C were measured according to Zhangjuntian's improved methods. The contents of mitochondrial calcium were detected by flame atom absorption. RESULTS: The contents of mitochondrial cytochrome C and calcium in model group were significantly lower than those in sham operation (P
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By means of a planimeter and skulls adjusted in the eye-ear plane, measurements of areas of the foramen ovale, spinosum, lacerum, jugulae and orifice of the canalis caroticus on both sides of the external surface of the skull base have been taken on 100 adult skulls collected in Chengdu. The symmetry of these areas has been studied. The main results of the study are given below: 1. In 4% of the whole series, the area of the foramen ovale is approximately equal on the two sides and the difference in area between the two sides is less than 1%. In 44%, the right side is larger, and in 52%, the left is larger. 2. In 5% of the whole series, the area of the foramen spinosum is approximately equal on the two sides. In 42%, the right side is larger, and in 53%, the left is larger. 3. In 4% of the whole series, the area of the foramen lacerum is approximately equal on the two sides. In 46%, the right side is larger, and in 50%, the left is larger. 4. In 3% of the whole series, the area of the foramen jugulae is approximately equal on the two sides. In 76%, the right side is larger, and in 21%, the left is larger. 5. In 3% of the whole series, the area of the anterior part of the foramen jugulae is approximately equal on the two sides. In 50%, the right side is larger, and in 47%, the left is larger. 6. In 17% of the whole series, the area of the intermediate part of the foramen jugulae is approximately equal on the two sides. In 37%, the right side is larger, and in 46%, the left is larger. 7. In 2% of the whole series, the area of the posterior part of the foramen jugulae is approximately equal on the two sides. In 79%, the right side is larger, and in 19%, the left is larger. 8. In 5% of the whole series, the area of the orifice of the canalis caroticus is approximately equal on the two sides. In 58%, the right side is larger, and in 37%, the left is larger. Moreover, a quantitative analysis of the bilateral areas has been carried out. The foramen area is highly positive in correlation With the product of its length and width. Therefore, the regression formulas of the areas are as follows: The foramen ovale-right, ■=1.5129+0.6699x (r=0.9417); left, ■=0.3695+0.6876x(r=0.8949). The foramen spinosum-right, ■=0.7827+0.6089x (r=0.8755); left, ■=0.3499+0.6695x (r=0.9207). The foramen lacerum-right, ■=9.4190+0.4480x (r=0.9147); left, ■=13.5198+0.4082x (r=0.8493). The foramen jugulaeright, ■=18.3913+0.5092x (r=0.8843); left,■=11.3561+0.5744x (r=0.9193). The anterior part of the foramen jugulae-right, Y=3.3141+0.4858x (r=0.8330); left, ■=3.2294+0.4854x (r=0.8452) The intermediate part of the foramen jugulae-right, ■=2.5434+0.4311x (r=0.7942); left, Y=1.7547+0.5227x (r=0.8366). The posterior part of the foramen jugulae-right,■=1.3470+0.6311x (r=0.8273); left, ■=0.9110+0.6586x (r=0.9480). The orifice of the canalis caroticus-right, ■=1.0986+0.6794x (r=0.9128); left, ■=1.5038+0.6590x (r=0.9219). (x is the product of the length and width of its foramen).